Effects of low level O₃ exposure on leaf diffusive conductance and water‐use efficiency in hybrid poplar

Abstract

Abstract Young, amphistomatous hybrid poplar ( Populus deltoides x trichocarpa ) plants were exposed daily to either background (0.025 cm 3 m ‐3 ) or elevated (0.125 cm 3 m ‐3 ) concentrations of O 3 . Levels of abaxial and adaxial leaf conductance were affected interactively by pollutant treatment, leaf age, and photon fluence rate. Consequently, conductance in O 3 ‐treated leaves was sometimes higher and sometimes lower than in comparable control leaves, depending on leaf age or level of photon fluence rate. For example, at low photon fluence rate or in the dark, conductance was greater in O 3 ‐treated than in control plants, while at high photon fluence rate that relationship was reversed. Exposure to O 3 also reduced the water‐use efficiency and range of leaf conductance of individual leaves, and altered the relationship between the conductances of the two leaf surfaces (the ratio of abaxial to adaxial leaf conductance was increased). Furthermore, O 3 treatment resulted in diminished stomatal control of water loss; excised O 3 ‐treated leaves had higher conductances and wilted sooner than excised control leaves of identical ages. Overall, the data indicate that exposure to O 3 resulted in impaired stomatal function.