INDUCTION OF CELL PROLIFERATION IN THE MOUSE BLADDER BY 4‐ETHYLSULPHONYL‐NAPHTHALENE‐1 ‐SULPHONAMIDE

Abstract

ABSTRACT The reaction of the bladder epithelium of mice, following stimulation with a carcinogen, 4‐ethylsulphonylnaphthalene‐1‐sulphonamide (ENS), was studied. A wave of cell division was induced in the resting epithelium, diploid and tetraploid cells being the main dividing elements; some of the high ploidy surface cells also underwent division. The cell cycle time for the diploid and tetraploid cells appeared to be identical. There was considerable variation in the intensity and timing of the onset of cell division in the bladder epithelium of individual animals. ENS caused severe damage to the surface cells of the bladder epithelium as indicated by increased lysosomes and the formation of large vacuoles.