<b>Dense immunostainings on both neuromuscular and myotendon junctions with an anti-dystrophin monoclonal </b><b>anfibody </b>

Abstract

Subcellular distribution of dystrophin in skeletal muscles was investigated by indirect immunofluorescence microscopy with an anti-dystrophin monoclonal antibody, AIC. The immunoreactivity was observed selectively on the surface membrane as reported previously (30). In addition, dense immunostainings on both neuromuscular and myotendon junctions were observed. The dense immunostaining on neuromuscular junction was well-demarcated, whereas that on myotendon junction was ill-defined in the margin and gradually decreased to the level of extrajunctional sarcolemma. The uneven distribution of dystrophin on sarcolemma is discussed with putative abnormalites of sarcolemmal elements found in Duchenne muscular dystrophy in view.