Variation of silica bodies in leaf epidermal long cells within and among seventeen species of <i>Oryza</i> (Poaceae)

Abstract

Morphometric procedures were used with scanning electron microscopy backscattered images to study silica bodies in epidermal long cells of four different leaf veins of 17 of the 20 species of Oryza. The veins studied were midrib, large vein, small vein, and marginal vein. Image analysis was used to study morphological variations among the silica bodies. Statistical analyses were based on 11 variables. Even within a single leaf, silica bodies were not uniform. However, the degree of morphological variation normally showed a distribution of morpholgical types around one modal shape. The most significant differences observed were between silica bodies of the midrib and those of other veins. Bodies varied with respect to both size and shape. Computer-assisted image analysis is an effective tool for categorizing basic data and for statistical analysis of variation among silica bodies. Morphological variation among silica bodies of a single leaf may be related to water-conducting systems and their influence on silica availability and phytolith formation.