Quantitative molecular and culture analyses of bacterial elimination in oval‐shaped root canals by a single‐file instrumentation technique

Abstract

The single-file technique was comparable with the conventional technique in oval-shaped canals provided the width of apical preparation, volume of irrigants and duration of irrigation are kept similar. No significant difference was observed for qPCR and culture in post-instrumentation samples, indicating that both methods can be reliably used for studies of antibacterial effectiveness.