Micro fluid segment technique for screening and development studies on Danio rerio embryos

Abstract

The applicability of micro fluid segments for studying the behaviour of multicellular systems, in particular embryonic development, has been investigated. It was found that eggs from the zebrafish Danio rerio can be introduced into micro fluid segments without serious damage by using perfluoromethyldecalin (PP9) as the carrier liquid and Teflon (PTFE) as the tube material. The development processes of fish embryos were observed over a time period of 80 hours, until hatching time. After five days, the fish larvae were brought out of the micro fluid segments and transferred into breeding reservoirs. Effects of the membrane-damaging anionic surfactant sodium dodecyl sulfate (SDS) alone and SDS with the addition of CuCl(2) (copper(II) chloride) were investigated. By analyzing different end points, we found inhibiting and also supporting effects on the development of the embryos. Low SDS concentrations with and without copper(II) ions were supportive, while higher SDS concentrations led to negative impacts on the development of the embryos. The results showed that automated micro screening processes with complex biological systems can be performed using microfluidic systems and are applicable for future toxicological and drug screening studies.