Microbial Adaptability to Organic Loading Changes in an Enhanced Biological Phosphorus Removal Process

Abstract

The enhanced biological phosphorus removal (EBPR) performances of phosphate-accumulating organisms (PAOs) under organic loading fluctuations were investigated using a sequencing batch reactor (SBR) with anaerobic/oxic stages. The adaptability of PAOs was evaluated after establishing a normal steady-state condition [chemical oxygen demand ( CODin )=150 mg/L]. During SBR operation, the initial COD was changed gradually or abruptly. When the initial COD increased gradually from the steady state to 300 mg/L, the biomass increased steadily and the system showed stable EBPR. However, when the initial COD oscillated from 150 to 300 or 50 mg/L abruptly, PAOs could not adapt themselves to these sudden changes, resulting in unstable EBPR. When the organic loading returned to a normal condition, the system was recovered to stable EBPR in 2 days after the high organic loading fluctuation, while it was not after the low organic loading fluctuation. Using fluorescent in situ hybridization technique, Rhodocyclus-related PAO population changes were monitored. It was demonstrated that PAOs would wash out faster under the low organic loading fluctuation than the high organic loading fluctuation.