Boron Nutrition of Cultured Tobacco BY-2 Cells. III. Characterization of the Boron-Rhamnogalacturonan II Complex in Cells Acclimated to Low Levels of Boron

Abstract

Cultured cells of tobacco (Nicotiana tabacum L.) BY-2 which could propagate at the same rate as the parent cells (1 mg B liter(-1)) under a lower level of boron (0.01 mg B liter(-1)) were obtained. The selected cells had swollen cell walls. In the parent cells, all the RG-II occurred as a B-RG-II complex, however, two-thirds of the RG-II occurred in a monomeric form in the selected cells.