Factors modifying regeneration in vitro of adventitious shoots in five red raspberry cultivars.

Abstract

A number of experiments were undertaken, using the leaves from four-week-old donor shoots of five raspberry cultivars, in an attempt to improve the process of regeneration of adventitious shoots in vitro. The cultivars used were ‘Beskid’, ‘Canby’, ‘Malling Seedling’, ‘Norna ’ and ‘Veten’. The medium for donor shoots of ‘Malling Seedling ’ and ‘Canby ’ should contain N6 mineral salts (Chu et al., 1975) and for the ‘Beskid’, ‘Norna ’ and ‘Veten’, Murashige and Skoog’s (1962) mineral salts, 0.6 mg l-1 6-benzylaminopurine and 0.1 mg l-1 indole-3-butyric acid. Murashige and Skoog’s medium supplemented with thidiazuron and indole-3-butyric acid (both at 0.1 mg l-1) and with FeEDTA replaced with FeEDDHA is recommended for regeneration. The medium pH for the regeneration of ‘Canby ’ and ‘Malling Seedling’ should be lowered to 4.6. The highest regeneration potential was observed in ‘Beskid’, and the lowest in ‘Malling Seedling’.