A General Approach for the Identification of Site‐Specific RNA Binders by ¹⁹ F NMR Spectroscopy: Proof of Concept

Abstract

Bound to succeed: Noncovalent binding of ligands to RNA can be analyzed easily by 19F NMR spectroscopy through site-specific fluorine-labeling of the target RNA (see picture; B=adenine, cytosine, guanine, uracil). The proof of concept is provided by RNA aptamer–ligand interactions. The speed and the robustness of this simple approach present major advantages over methods relying on 1H NMR spectroscopy. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z504174_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.