A Newly Designed Experimental System for Exposure of Mammalian Cells to Extremely Low Frequency Magnetic Fields.

TLDR

The study designed a new system for long‑term, high‑density exposure of mammalian cells to extremely low frequency magnetic fields to investigate their biological effects. The system comprises a magnet generator with a built‑in CO₂ incubator, AC power supply, gas compressor, thermocontroller, and cooling unit, maintaining 37 ± 0.5 °C and delivering up to 500 mT at 50 Hz. Long‑term exposure to 400 mT ELFMF for up to 120 h did not affect growth of HL60RG and CCRF‑CEM leukemia cells, nor did 2 h post‑X‑irradiation exposure alter radiation sensitivity of GM0637 and TAT2SF cells.

Abstract

To examine the biological effects of extremely low frequency magnetic field (ELFMF), we have designed and manufactured a new equipment for long-term and high-density exposure of cells to ELFMF. The ELFMF exposure system consists of a generator of magnets with a bult-in CO2 incubator, an alternating current (AC) power supply, a gas compressor and a thermocontroller for the incubator, and a cooling unit for the magnets. The CO2 incubator made of acrylic resin is inserted into the inner-space of the silicon steel strip-cores. In this system, the temperature of the incubator is maintained at 37±0.5°C. The maximum magnetic flux density on the exposure area of the incubator is 500 mT (T; tesla) at a current of 556 Arms (rms; root mean square) at 50 Hz. The long-term (up to 120 hr) exposure of 400 mT ELFMF did not affect the growth of both HL60RG and CCRF-CEM cells originated from human leukemia. The post-X-irradiation exposure of 400 mT ELFMF for 2 hr also did not affect the radiation sensitivity of GM0637 and TAT2SF cells originated from a normal human and an ataxia telangiectasia patient.