Quantum Dot/Bioluminescence Resonance Energy Transfer Based Highly Sensitive Detection of Proteases

Abstract

BRET als Erkennungsmerkmal: Quantenpunkt(QD)-Nanosensoren detektieren die Aktivität von Matrixmetalloprotease, indem sie die Effizienz des resonanten Biolumineszenzenergietransfers (BRET) zwischen dem QD und einem biolumineszierenden Fusionsprotein erfassen (siehe Schema; Luc8=Renilla-Luciferase; His×6=Hexahistidin-Marker). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2007/z700280_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.