Revisions in the technique of epi-illumination light microscopy for the study of floral and vegetative apices

Abstract

Many improvements have been made in the technique of epi-illumination light microscopy. These modifications have resulted in micrographs of better resolution and subsequently in greater flexibility in the study of floral and vegetative apices. The preparation, mounting, microscopy, and photography of apices are discussed in detail. The correlation of this technique with others such as scanning electron microscopy and serial sectioning is discussed. The numerous applications of epi-illumination light microscopy are considered.