Concepedia

PRINS, the other in situ DNA labeling method useful in cellular biology

Philippe Coullin, Lydia Roy, Franck Pellestor, Jean‐Jacques Candelier, Bertrand Bed’Hom, Z. Guillier-Gencik, Alain Bernheim

American Journal of Medical Genetics · 2001 · 22 citations · 27 references

DOI

Concepts

Abstract

PRINS has proven to be an attractive alternative to FISH for in situ DNA labeling. PRINS is specific, simple, and rapid. We review some applications of PRINS involving primers specific for telomeric, human Alu, and centromeric alpha-satellite sequences. Bicolor labeling, PRINS-FISH, or PRINS-immunofluorescent combinations have been developed to enable investigations in numerous applications.

References

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Richard Saffery · Human Molecular Genetics · 2000 · 194 citations · Full text

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