Role of volatile semiochemicals in host location by the egg parasitoid <i><scp>A</scp>nagrus breviphragma</i>

Abstract

Abstract Recent investigations conducted on several tritrophic systems have demonstrated that egg parasitoids, when searching for host eggs, may exploit plant synomones that have been induced as a consequence of host oviposition. In this article we show that, in a system characterized by host eggs embedded in the plant tissue, naïve females of the egg parasitoid A nagrus breviphragma S oyka ( H ymenoptera: M ymaridae) responded in a Y ‐tube olfactometer to volatiles from leaves of C arex riparia C urtis ( C yperaceae) containing eggs of one of its hosts, C icadella viridis ( L .) ( H emiptera: C icadellidae). The wasp did not respond to host eggs or to clean leaves from non‐infested plants compared with clean air, whereas it showed a strong preference for the olfactometer arm containing volatiles of leaves with embedded host eggs, compared with the arm containing volatiles of leaves from a non‐infested plant or host eggs extracted from the plant. When the eggs were removed from an infested leaf, the parasitoid preference was observed only if eggs were added aside, suggesting a synergistic effect of a local plant synomone and an egg kairomone. The parasitoid also responded to clean leaves from an egg‐infested plant when compared with leaves from a non‐infested plant, indicating a systemic effect of volatile induction.