Automated Immunoturbidimetric Method for Measuring Serum Transferrin Receptor

Abstract

6% of the differences in immunoactivity among rcTnI-0, rcTnI-6, and rcTnI-14 can be attributed to the use of the Bradford assay. The observed immunoactivity differences among rcTnI-0, rcTnI-6, and rcTnI-14 were much greater than 6%. In the Access assay, immunoactivity was markedly decreased if a leader sequence was present in the TnI molecule. It also seemed that the extent to which immunoactivity was decreased was related to the length of the leader sequence. This tendency was not observed in the Stratus assay, indicating that the presence of leader sequence may affect only certain epitopes and, therefore, certain assays.