Journal of Medical Microbiology · 1991 · 19 citations · 14 references
Life SciencesMicrobiologyMolecular GeneticsBacteriologyMedical MicrobiologyMicrobial GeneticsBacterial GeneticsBacterial IdentificationDiagnostic BacteriologyGenotypic CharacterizationMolecular TypingStrain TypingBacterial TypingLegionella InfectionMolecular Identification Of StrainsMolecular Typing Of BacteriaMolecular Typing Of IsolatesMolecular Typing Of PathogensRestriction Fragment Length Polymorphism AnalysisRibosomal Dna Analysis
The use of probes derived from rRNA sequences to detect restriction fragment length polymorphisms (RFLPs) associated with the ribosomal RNA genes for epidemiological typing (ribotyping) is a powerful and readily applicable tool. Different probes and enzymes for ribotyping were compared for a series of 73 unrelated Legionella pneumophila serogroup 1 strains. The probes compared were cDNAs, transcribed from L. pneumophila or Escherichia coli rRNA subunits, and a cloned L. pneumophila rRNA gene. The cloned rRNA gene probe gave the best discrimination and this probe was further compared with cloned probes comprised of randomly selected (non-rRNA) parts of the L. pneumophila chromosome. In this instance the greatest discrimination was achieved when one of the non-ribosomal RNA gene probes was employed. The overall discrimination of RFLP typing was enhanced by combining the data obtained with both rRNA and non-rRNA probes.
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