In vitro Evaluation of the Efficacy of Leaf and its Callus Extracts of Cardiospermum halicacabum L. On Important Human Pathogenic Bacteria

Abstract

The investigation is aimed to carry out the antimicrobial activities of different solvent extracts of
\nleaf and leaf derived callus extracts of Cardiospermum halicacabum L. (Sapindaceae). The leaf segments of
\nC. halicacabum were cultured on MS medium supplemented with auxins and cytokinins alone. Maximum callus
\nwas recorded on medium containing BAP. The antibacterial properties of leaf and its callus extracts were
\nscreened against ten human pathogenic bacteria by cup diffusion method. Powdered leaf and leaf derived callus
\nmaterial was subjected to extraction of aqueous and with different organic solvents viz., petroleum ether,
\nchloroform, methanol and ethanol using soxhlet apparatus. All the solvent extracts were evaporated to dryness
\nusing rotary flash evaporator. Dry residues was dissolved in methanol, and tested for antibacterial activity.
\nAmong all the solvents tested, significant inhibitory activity was observed in ethanol extract of both leaf and
\nleaf derived callus followed by methanol. It also observed that the activity was more pronounced on
\nGram-positive bacteria than Gram-negative bacteria.