Cauliflower Mosaic Virus: an Improved Purification Procedure and Some Properties of the Virus Particles

Abstract

SUMMARY A new procedure for the purification of cauliflower mosaic virus (CaMV) which involves the use of Triton X-100 and urea results in higher and more reliable yields. CaMV particles have a s 20, w of 208S, a D 20, w of 0µ75 × 10-7 cm2/s, contain 17% nucleic acid and have a mol. wt. of 22.8 × 106. Virus precipitated by Mg2+, low pH or ethanol at moderate salt concentrations, or by polyethylene glycol, is not easily resuspended.