American Journal of Tropical Medicine and Hygiene · 2012 · 49 citations · 28 references
Veterinary MedicineInfectious DiseasesMicrobiologyDiagnostic MicrobiologyMedical MicrobiologyParasitologyHelminth InfectionsMolecular Diagnosis Of ParasitesParasitic DiseasesParasitic ZoonosesZoonotic HelminthsZoonotic Parasitic DiseasesHookworm ZoonosesInternal Transcribed Spacer SequencingMolecular Detection Of ParasitesMolecular Diagnosis Of Parasitic InfectionsMolecular Species IdentificationParasitic Zoonotic DiseasesSequence ComparisonZoonotic Transmission Pathways
Species identification of human hookworm infections among eight communities in rural areas of Peninsular Malaysia was determined during 2009-2011. Fecal samples were examined by microscopy and subsequently, the internal transcribed spacer 2 and 28S ribosomal RNA region of Necator americanus and Ancylostoma spp. were sequenced. Overall, 9.1% (58 of 634) were identified positive by microscopy for hookworm infection, and 47 (81.0%) of 58 were successfully amplified and sequenced. Sequence comparison found that N. americanus (87.2%) was the most predominant hookworm identified, followed by Ancylostoma ceylanicum (23.4%). No A. duodenale infection was detected in this study. Detection of A. ceylanicum in humans highlighted the zoonotic transmission among humans living near dogs. Thus, implementation of effective control measures for hookworm infections in future should seriously consider this zoonotic implication.
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MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
Koichiro Tamura, Joel T. Dudley, M Nei et al. · Molecular Biology and Evolution · 2007 · 28.8K citations · Full text
Rapid sequencing of rDNA from single worms and eggs of parasitic helminths
Robin B. Gasser, Neil B. Chilton, Hervé Hoste et al. · Nucleic Acids Research · 1993 · 551 citations