Rapid Communications in Mass Spectrometry · 1998 · 50 citations · 0 references
PharmacyBiochemistryAnalytical ChemistryMass Spectrometric AnalysisSequence AnalysisChromatographic CouplingChromatography CouplingDe Novo Peptide SequencingMass SpectrometryPeptide AnalysisPeptide IdentificationIon Trap Mass SpectrometryIsotopic Labeling Of PeptidesLiquid Chromatography-mass SpectrometryPeptide Mass SpectrometryPeptide SequencingQuadrupole Mass SpectrometryTime-of-flight Mass AnalyzersTime-of-flight Mass Spectrometry
De novo peptide sequencing in an ion trap mass spectrometer coupled on-line with a capillary HPLC using 18O labeling provides a viable alternative to the method using the combination of nanospray, 18O labeling and a quadrupole/time-of-flight mass spectrometer. Seven to sixteen amino acid residues can be sequenced from the liquid chromatography/randem mass spectrometry (LC/MS/MS) spectra. This approach combines the benefit of capillary LC and the high sensitivity of the ion trap operated in the MS/MS mode. The wide availability of the LCQ mass spectrometer makes this approach readily adaptable to the biological mass spectrometry community.