Ratiometric Molecular Sensor for Monitoring Oxygen Levels in Living Cells

Abstract

A ratiometric oxygen sensor (C343-Pro4-BTP) that uses coumarin 343 (blue in picture) as an oxygen-insensitive fluorophore and an iridium complex (BTP, red in picture) as an oxygen-sensitive phosphor can be used to determine the oxygen concentration in solution and in living cells, which was demonstrated by ratiometric measurements of the blue coumarin 343 fluorescence and the red BTP phosphorescence in C343-Pro4-BTP (see picture). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.