A technique to collect and dislodge conidia produced by<i>Paracoccidioides brasiliensis</i>mycelial form

Abstract

Collection and separation of mycelial propagules produced by P. brasiliensis was accomplished by agitation with glass beads, centrifugation and filtration through cotton wool. The mean number of conidia liberated per plate (approximately 1 000 000) and their viability (79%), lead us to think that it is now possible to undertake experimental studies with these propagules.