An Autoimmolative Spacer Allows First‐Time Incorporation of a Unique Solid‐State Fluorophore into a Detection Probe for Acyl Hydrolases

Abstract

Mind your HPQs: A probe to detect specific peptidases based on the unique solid-state fluorophore HPQ has been developed by the use of an autoimmolative cyclization spacer in conjunction with an acylated O,O-acetal linked to HPQ (see scheme). Under our assay conditions this probe detected the enzyme with a minimum half-time of only 560 s. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.