Journal of High Resolution Chromatography · 1988 · 15 citations · 12 references
PharmacyPharmaceutical AnalysisPharmacologyDrug MetabolismMass Spectrometric AnalysisSteroid BiochemistryDrug DispositionMetabolite IdentificationSteroid PharmacologyToxicological Screening Of PharmaceuticalsCapillary Gas ChromatographyLiquid Chromatography-mass SpectrometryLiquid Chromatography-mass Spectrometry AnalysisMetabolite PharmacokineticsMicrocolumn ChromatographySteroid Metabolite AnalysisSteroid Metabolite ProfilingUrinary Steroid Analysis
Abstract Adult male volunteers were administered orally 10 mg of the espective drug. Urine samples were investigated by micro‐column liquid chromatography and capillary gas chromatography combined with on‐line mass spectrometry. The former technique was found to be usable for the detection of these drugs. The latter method appeared to be superior in terms of sensitivity and for metabolism studies. The major excretion products of dexamethasone were identified as isomeric 6‐hydroxy metabolites. Dexamethasone as such and its 20‐hydroxy metabolite were found in minor quantities. Unlike dexamethasone, triamcinolone is excreted largely unchanged from the human body. Two metabolites were identified as 11‐keto and 4,5‐dihydrotriamcinolone. With the gas chromatography methods developed, abuse of these drugs can be detected up to 24 h after administration of a 5 mg single dose.
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