Protein‐Phosphorylation‐Responsive Polymeric Nanoparticles for Imaging Protein Kinase Activities in Single Living Cells

Abstract

Take your PIC: Cell-permeable and biocompatible polyion-induced complex (PIC) nanoparticles were developed consisting of a negatively charged polymer along with a positively charged polymer conjugated to a near-infrared fluorophore and a protein-kinase-specific peptide. Owing to profound changes in fluorescence, the PIC nanoparticle is an attractive probe for protein kinase A (PKA) in single living cells. ATP=adenosine triphosphate, ADP=adenosine diphosphate.