The preparation of ⁵⁹ Fe-labelled transferrin for ferrokinetic studies

Abstract

Serum labelled with (59)Fe for ferrokinetic studies should contain only [(59)Fe] transferrin. [(59)Fe] ferric citrate was incubated at 37 degrees C for up to 24 hours with sera having an excess latent iron-binding capacity. The results showed that unbound [(59)Fe] ferric citrate may be present for several hours. An anion exchange resin (IRA 400 Cl(-)) column removed all the [(59)Fe] ferric citrate and left only [(59)Fe] transferrin in the eluate. It is suggested that all (59)Fe-labelled preparations for ferrokinetic studies should be passed through a resin column before injection.