Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

Andrew P. Feinberg, Bert Vogelstein · Analytical Biochemistry · 1983 · 25.2K citations

Nucleic Acid Chemistry, High Specific Activity, Natural Sciences +6

Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels

Michael McDonell, Martha N. Simon, F. William Studier · Journal of Molecular Biology · 1977 · 1.6K citations

Dna Sequencing, Restriction Fragments, Natural Sciences +12

Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

Val C. Sheffield, David R. Cox, Leonard S. Lerman et al. · Proceedings of the National Academy of Sciences · 1989 · 1.3K citations · Full text

Genetics, Radioactive Probes, Dna Analysis +18

pEMBL: a new family of single stranded plasmids

Luciana Dente, Gianni Cesareni, Riccardo Cortese · Nucleic Acids Research · 1983 · 1.2K citations · Full text

Genetics, Molecular Biology, Molecular Genetics +16

[31] Detection and localization of single base changes by denaturing gradient gel electrophoresis

R Myers, Tom Maniatis, Leonard S. Lerman · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 935 citations

Engineering, Capillary Electrophoresis, Biochemistry +11