Human Hydration Indices: Acute and Longitudinal Reference Values

It is difficult to describe hydration status and hydration extremes because fluid intakes and excretion patterns of free-living individuals are poorly documented and regulation of human water balance is complex and dynamic. This investigation provided reference values for euhydration (i.e., body mass, daily fluid intake, serum osmolality; M ± SD ); it also compared urinary indices in initial morning samples and 24-hr collections. Five observations of 59 healthy, active men (age 22 ± 3 yr, body mass 75.1 ± 7.9 kg) occurred during a 12-d period. Participants maintained detailed records of daily food and fluid intake and exercise. Results indicated that the mean total fluid intake in beverages, pure water, and solid foods was >2.1 L/24 hr (range 1.382–3.261, 95% confidence interval 0.970–3.778 L/24 hr); mean urine volume was >1.3 L/24 hr (0.875–2.250 and 0.675–3.000 L/24 hr); mean urine specific gravity was >1.018 (1.011–1.027 and 1.009–1.030); and mean urine color was ≥4 (4–6 and 2–7). However, these men rarely (0–2% of measurements) achieved a urine specific gravity below 1.010 or color of 1. The first morning urine sample was more concentrated than the 24-h urine collection, likely because fluids were not consumed overnight. Furthermore, urine specific gravity and osmolality were strongly correlated ( r 2 = .81–.91, p < .001) in both morning and 24-hr collections. These findings provide euhydration reference values and hydration extremes for 7 commonly used indices in free-living, healthy, active men who were not exercising in a hot environment or training strenuously.