Genetic transformation of a local variety of tomato using gus gene: an efficient genetic transformation protocol for tomato.

Abstract

Institute of Agri - Biotechnology, Department of Biotechnology, University of Agricultural Sciences, Dharwad – 580 005, Karnataka, India. Paramesh, H., Fakrudin, B. and Kuruvinashetti, M.S. (2010). Genetic transformation of a local variety of tomato using gus gene: an efficient genetic transformation protocol for tomato. Journal of Agricultural Technology 6(1): 87-97. An efficient and reproducible transformation protocol was optimized for L15, a local tomato variety using Agrobacterium strain GV 2260 carrying β-glucoronidase gene on pCAMBIA 1301 plasmid with hpt I gene as a plant selection marker. The use of pre-cultured explants (cotyledon leaf and hypocotyl) helped not only in easy handling of explants during transformation experiments, but also in reducing the Agrobacterium contamination and death before callusing due to insufficient strength of explants to bear the antibiotic treatments. Agrobacterium cell density (OD