Multiplex PCR for bla(CTX-M) & bla(SHV) in the extended spectrum beta lactamase (ESBL) producing gram-negative isolates.

Abstract

Our study demonstrated rapid detection of bla(SHV) and bla(CTX-M) in isolates belonging to Enterobacteriaceae and other non-fermenting clinical isolates using multiplex PCR. This genotypic method provided a rapid and efficient differentiation of ESBLs in the laboratory.