IN VITRO EFFECT OF METHISOPRINOL ON SALMONID RHABDOVIRUSES REPLICATION

Abstract

The in vitro influence of methisoprinol on the replication of viral haemorrhagic septicaemia (VHS) and infectious haematopoetic necrosis (IHN) viruses by measuring their RNA synthesis was examined. EPC cell cultures containing various concentrations of methisoprinol (0, 100, 200, 300, 400 and 500 μg/ml of medium) were inoculated with VHS or IHN virus suspension containing 10 TCID50/ml. The inhibition of [H]-uridine incorporation into VHSV and IHNV RNA propagated in cell cultures with methisoprinol was demonstrated. The highest percentage of viral RNA inhibition was observed when a dose of 500 μg/ml was used. The results indicated that methisoprinol inhibits the replication of salmonid rhabdoviruses and possibly may be effective in prevention of viral infection in aquaculture.