A Nucleoside That Contains a Rigid Nitroxide Spin Label: A Fluorophore in Disguise

Abstract

Take a spin: The nucleoside Ç (C-spin), which contains a rigid nitroxide spin label, allows the structure and dynamics of nucleic acids into which it is incorporated to be studied by EPR spectroscopy (left spectrum). Reduction of the nitroxide with a mild reducing agent renders the nucleoside fluorescent (right spectrum). Thus, the same nucleic acid can be studied with two complementary spectroscopic techniques. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z603993_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.