A Colorimetric Method for Assay of Erythrocytic Glucose-6-phosphate Dehydrogenase.

Abstract

SummaryA colorimetric method for estimation of glucose-6-phosphate dehydrogenase activity of erythrocytic hemolysates is described. The method reported employs phenazine methosulfate as an electron carrier between TPNH formed in the reaction and dichloroindophenol. Rate of reduction of the latter compound is followed spectrophotometrically. The method gives turnover rates comparable to those obtained with the ultraviolet spectrophotometric method and is more sensitive than the latter. The assay utilizes a standard spectrophotometer or photoelectric colorimeter and is independent of diaphorase activity. The method should be readily adaptable to visual estimation of glucoses-phosphate dehydrogenase of erythrocytes.