M2 Proton Channel Structural Validation from Full‐Length Protein Samples in Synthetic Bilayers and <i>E. coli</i> Membranes

Abstract

Validation: Membrane protein structures are sensitive to the environment used for structural characterization. NMR spectra of the full-length M2 proton channel from influenza A were measured directly in E. coli membranes and compared to spectra of the protein in synthetic lipid bilayers. The results demonstrate that these bilayers provide a native-like membrane environment.