Crystalline degradation products of vancomycin as chiral stationary phase in microcolumn liquid chromatography

Abstract

The ability of crystalline degradation products (CDPs) of vancomycin as a chiral stationary phase was reported in a previous study for enantioselective separation of drugs, amino acids and agrochemical toxins by conventional LC column (250 x 4.6 mm). In this work, the potential of CDP of vancomycin for the enantiomeric separation in micro-LC (200 x 1 mm) has been studied. The obtained separation results are better than in our previous study with conventional LC columns. The enantiomers of D,L-phenylalanine, D,L-alanine, methyldopa, atropine and propranolol were used for this evaluation. Experiments have been carried out in a stainless steel tube that was packed with chiral silica particles of 3 and 12 microm diameters. Also, three different ratios of 3 and 12 microm silica particles were used for packing material of chiral columns and the effect on aspect ratio and resolving powers was compared.