ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins

TLDR

ScanProsite is a web-based tool that matches protein sequences to PROSITE signatures and ProRules, supports user‑defined and UniProtKB sequences, uses a precomputed match database for speed, and offers multiple output modes including an interactive graphical viewer. Incorporating ProRules into signature matching improves the accuracy of function prediction based on profiles.

Abstract

ScanProsite— http://www.expasy.org/tools/scanprosite/ —is a new and improved version of the web-based tool for detecting PROSITE signature matches in protein sequences. For a number of PROSITE profiles, the tool now makes use of ProRules—context-dependent annotation templates—to detect functional and structural intra-domain residues. The detection of those features enhances the power of function prediction based on profiles. Both user-defined sequences and sequences from the UniProt Knowledgebase can be matched against custom patterns, or against PROSITE signatures. To improve response times, matches of sequences from UniProtKB against PROSITE signatures are now retrieved from a pre-computed match database. Several output modes are available including simple text views and a rich mode providing an interactive match and feature viewer with a graphical representation of results.