Relevance of microbial extracellular polymeric substances (EPSs) - Part I: Structural and ecological aspects

TLDR

Extracellular polymeric substances, composed mainly of polysaccharides, proteins, and lipids, form the matrix of microbial aggregates such as biofilms and sludge, enabling stable multicellular consortia, retention of enzymes and nutrients, light transmission in photosynthetic mats, and dynamic responses to environmental changes. In the EPS matrix, physiological activity creates gradients because diffusion dominates over convection. Biofilm cells tolerate higher concentrations of many biocides.

Abstract

Extracellular polymeric substances are the construction materials for microbial aggregates such as biofilms, flocs ("planktonic biofilms") and sludge. Their major components are not only polysaccharides but also proteins and in some cases lipids, with minor contents of nucleic acids and other biopolymers. In the EPS, biofilm organisms can establish stable arrangements and function multicellularly as synergistic microconsortia. The matrix facilitates the retention of exoenzymes, cellular debris and genetic material; it can be considered as a microbial recycling yard. Gradients can develop due to the physiological activity and the fact that diffusive mass transport prevails over convective transport in the matrix. Biofilm cells tolerate higher concentrations of many biocides. The EPS matrix sequesters nutrients from the water phase. In photosynthetic communities, EPS molecules can function as light transmitters and provide photons to organisms located deeper in a microbial mat. The EPS matrix is a dynamic system, constructed by the organisms and responding to environmental changes. It enables the cells to function in a manner similar to multicellular organisms.