The Cofactor of the Iron–Sulfur Cluster Free Hydrogenase Hmd: Structure of the Light‐Inactivation Product

Abstract

A combination of NMR spectroscopy and mass spectrometry has been crucial in determining the structure of the relatively stable light-inactivated form of the cofactor of the hydrogenase Hmd (see scheme). These studies have shown that the chromophore bound to GMP through a phosphoester linkage is a pyridone derivative.