Journal of Virology · 1981 · 63 citations · 18 references
Life SciencesInfectious DiseasesMolecular BiologyMolecular VirologyMonoclonal AntibodiesVirologyRna-binding ProteinsViral Gene ExpressionViral Rna BiologyViral StructureViral TranscriptionVirus StructureCompetitive Binding AssaysCompetitive Enzyme-linked Immunosorbent AssayInfluenza Virus TranscriptionRna Virus BiologyRna-binding DomainsStructural Basis Of AntigenicityViral Rna TranscriptionViral Transcription Mechanisms
Monoclonal antibodies were used to map the antigenic domains of the A/WSN/33 (HoN1) influenza virus nucleoprotein. Three nonoverlapping antigenic regions of the nucleoprotein were identified by using competitive-binding enzyme-linked immunosorbent assays. Monoclonal antibodies to two nucleoprotein domains inhibited in vitro transcription of viral RNA, suggesting that these specific regions of the nucleoprotein are topographically or functionally involved in RNA transcription.
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