Electrical stimulation of nucleus raphe dorsalis changes morphine self-administration and withdrawal symptoms in rats

Abstract

The involvement of antero-dorsal part of the nucleus raphe dorsalis (NRD) in motivational aspects of drug-taking behaviour during initiation of drug self-administration was investigated using a recently developed behavioural paradigm. In separate experiments animals were allowed to self-administer morphine (1 mg/kg per inf) ten consecutive daily 3-h sessions. During all morphine self-administration sessions lever-press behaviour was measured in absence of electrical stimulation of NRD, as an index of the motivational aspects involved in drug-taking behaviour. The electrical stimulation (pulse 0.5 ms, 150 &mgr;A, 20 Hz) of NRD 30 min before morphine self-administration produced a significant decrease in the initiation of morphine self-administration during all sessions (reduced number of lever-press behaviour). After the last test session, morphine withdrawal syndrome signs (wet dog shakes, jumping, writhing and diarrhoea) in the naloxone-induced behaviour were measured. Our results showed that these withdrawal syndrome signs decreased by application of electrical stimulation in NRD, in comparison with morphine groups. It is concluded that serotonergic system in the NRD might be involved in the motivational processes underlying morphine self-administration.