An Improved Medium for the Rapid Growth of Isolated Potato Buds

Abstract

As a result of varying several components, a medium has been developed which sustains fast continuous growth of small potato buds in tissue culture. Growth-rates were increased with high concentrations of inorganic nutrients. Gibberellic acid and 8-hydroxyquinoline were required for the maintenance of the stem organization. In the absence of gibberellic acid the apical meristem degenerated to a callus. Without 8-hydroxyquinoline growth stopped when the stem was 6–10 mm long. Growth was faster following addition of indole-3-acetic acid.