DNA‐Based Carbohydrate Biochips: A Platform for Surface Glyco‐Engineering

Abstract

When the chips are down: A glycoarray consisting of oligonucleotide/carbohydrate conjugates hybridized onto a DNA chip can be used for the immobilization of oligosaccharides at extremely low concentrations (1 μM). With lectin, the lower detection limit of the device was 2–20 nm. The relative surface density of the conjugates was monitored by Cy3 fluorescence. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z604955_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.