Method for Regulated Expression of Single-Copy Efflux Pump Genes in a Surrogate <i>Pseudomonas aeruginosa</i> Strain: Identification of the BpeEF-OprC Chloramphenicol and Trimethoprim Efflux Pump of <i>Burkholderia pseudomallei</i> 1026b

Abstract

Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump.