High performance liquid chromatography (HPLC) of natural products. V. The use of HPLC in the cell-free biosynthetic conversion of .ALPHA.-aminoadipyl-cysteinyl-valine (LLD) into isopenicillin N.

Abstract

Isopenicillin N (I) and penicillin N (II) were separated effectively by reversed phase HPLC as derivatives of chiral 2, 3, 4, 6-tetra- O-acetyl-β-D-glucopyranosyl isothiocyanate (III). This permitted identification of isopenicillin N isolated by HPLC during a biosynthetic cell-free experiment using α-L-aminoadipyl-L-cysteinyl-D-valine (IV) as precursor.