Cancerous stem cells can arise from pediatric brain tumors

TLDR

Pediatric brain tumors are major causes of morbidity and mortality, and are thought to arise from self‑renewing multipotent neural stem cells. The study tested whether medulloblastomas, gliomas, and other pediatric brain tumors contain cells with neural stem‑cell properties. Tumor cells were isolated, cultured at clonal density to form neurospheres, and characterized for self‑renewal, multipotency, and differentiation under defined conditions. Tumor‑derived neurospheres self‑renew, are multipotent, express stem‑cell markers, can differentiate abnormally, migrate and proliferate in vivo, indicating that pediatric brain tumors harbor altered neural stem‑like cells that may drive tumorigenesis and present therapeutic targets.

Abstract

Pediatric brain tumors are significant causes of morbidity and mortality. It has been hypothesized that they derive from self-renewing multipotent neural stem cells. Here, we tested whether different pediatric brain tumors, including medulloblastomas and gliomas, contain cells with properties similar to neural stem cells. We find that tumor-derived progenitors form neurospheres that can be passaged at clonal density and are able to self-renew. Under conditions promoting differentiation, individual cells are multipotent, giving rise to both neurons and glia, in proportions that reflect the tumor of origin. Unlike normal neural stem cells, however, tumor-derived progenitors have an unusual capacity to proliferate and sometimes differentiate into abnormal cells with multiple differentiation markers. Gene expression analysis reveals that both whole tumors and tumor-derived neurospheres express many genes characteristic of neural and other stem cells, including CD133, Sox2 , musashi-1, bmi-1 , maternal embryonic leucine zipper kinase, and phosphoserine phosphatase, with variation from tumor to tumor. After grafting to neonatal rat brains, tumor-derived neurosphere cells migrate, produce neurons and glia, and continue to proliferate for more than 4 weeks. The results show that pediatric brain tumors contain neural stem-like cells with altered characteristics that may contribute to tumorigenesis. This finding may have important implications for treatment by means of specific targeting of stem-like cells within brain tumors.