SIMULTANEOUS DETERMINATION OF ACETAMINOPHEN AND TRAMADOL BY SECOND DERIVATIVE SPECTROPHOTOMETRY

Abstract

A rapid method for the simultaneous determination of acetaminophen (A) and tramadol (T) by second derivative spectrophotometric has been developed. From a solvent effect studies and the spectral behaviours of A and T, ethanol was selected as solvent. For a value of 210 nm a smoothing factor of 8,000 and scale factor of 1,000,000 were selected, because in these conditions the signal/noise ratio was favoured in order to avoid error. In these conditions also it is possible the simultaneous determination of A/T in a molar relation of 17/1 contained in pharmaceutical formulations. At 285.7 nm the second derivative value is T concentration dependent, corresponding to zero-crossing point of A. On the other hand, T does not absorb between 296.0 to 400.0 nm, thus 308.0 nm was selected for A determination by graphic method. The determination ranges for A and T were 8.1x10 -7 -51x10 -5 mol/L and 3.4 x10 -7 -5.0 x10 -5 mol/L, respectively and can be determined with good precision and accuracy, without previous separation. A study of interferents, was carried out by using the excipients Zafin tablet. The recoveries were 97.4 2.0% and 100.6 1.7% for A and T, respectively. This tablet containing both drugs was assayed using the methods developed, showing a good accuracy and precision.