Concepedia

Gram Type-Specific Broad-Range PCR Amplification for Rapid Detection of 62 Pathogenic Bacteria

Alfred Klausegger, Markus Hell, Alexandra Berger, Kerstin Zinober, Sabine Baier, Neil Jones, Wolfgang Sperl, Barbara Kofler

Journal of Clinical Microbiology · 1999 · 79 citations · 8 references

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Abstract

Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.

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