Adjustable Amplification of Synaptic Input in the Dendrites of Spinal Motoneurons <i>In Vivo</i>

TLDR

Investigate how neuromodulators alter active dendritic conductances in adult cat spinal motoneurons. Use intracellular recordings and somatic voltage clamp to estimate dendritic amplification of Ia afferent input. In decerebrate cats, Ia input was amplified up to sixfold by neuromodulatory activity, whereas in pentobarbital‑anesthetized preparations the current was reduced or only modestly increased; spinal transection abolished amplification, indicating that dendritic amplification is essential for driving slow‑fiber motoneurons to maximal force with minimal synaptic input and can be tuned by neuromodulators to meet task demands.

Abstract

The impact of neuromodulators on active dendritic conductances was investigated by the use of intracellular recording techniques in spinal motoneurons in the adult cat. The well known lack of voltage control of dendritic regions during voltage clamp applied at the soma was used to estimate dendritic amplification of a steady monosynaptic input generated by muscle spindle Ia afferents. In preparations deeply anesthetized with pentobarbital, Ia current either decreased with depolarization or underwent a modest increase at membrane potentials above −40 mV. In unanesthetized decerebrate preparations (which have tonic activity in axons originating in the brainstem and releasing serotonin or norepinephrine), active dendritic currents caused strong amplification of Ia input. In the range of −50 to −40 mV, peak Ia current was over four times as large as that in the pentobarbital-anesthetized preparations. Exogenous administration of a noradrenergic agonist in addition to the tonic activity further enhanced amplification (sixfold increase). Amplification was not seen in preparations with spinal transections. Overall, the dendritic amplification with moderate or strong neuromodulatory drive was estimated to be large enough to allow the motoneurons innervating slow muscle fibers to be driven to their maximum force levels by remarkably small synaptic inputs. In these cells, the main role of synaptic input may be to control the activation of a highly excitable dendritic tree. The neuromodulatory control of synaptic amplification provides motor commands with the potential to adjust the level of amplification to suit the demands of different motor tasks.