A Kinetic Photometric Method for Serum γ-Glutamyl Transpeptidase

TLDR

The study presents a kinetic photometric method to measure serum γ‑glutamyl transpeptidase activity. The method uses γ‑glutamyl‑p‑nitroanilide in 0.1 mL serum with an ammediol‑HCl buffer at pH 8.2, and an automated cuvette positioner, recorder, and diluter to perform over 60 determinations per hour. The method proved technically excellent and clinically valuable based on extensive testing.

Abstract

Abstract A kinetic method is described for the determination of γ-glutamyl transpeptidase activity in serum. Optimal conditions for the reaction were ascertained, normal values in milliunits per milliliter at 25° established, and the reliability of the method examined. γ-Glutamyl-p-nitroanilide in ammediol-HCl buffer at pH 8.2 is employed as substrate. The method requires only 0.1 ml. of serum and, using an automatic cuvet positioner and recorder as well as an automatic diluter, more than 60 determinations can be carried out in 1 hr. On the basis of a large number of determinations, the method was shown to be technically excellent and clinically valuable.